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| Registros recuperados: 68 | |
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| Dang, Cecile; De Montaudouin, Xavier; Binias, Cindy; Salvo, Flora; Caill-milly, Nathalie; Bald, Juan; Soudant, Philippe. |
| Perkinsosis is one of the most widespread diseases affecting commercially important species of molluscs globally. We examined the impact of Perkinsus spp. on shell growth at the individual scale in 2 clam species: Ruditapes decussatus from Mundaka Estuary (Spain) and R. philippinarum from Arcachon Bay (France). At Arcachon, 2 contrasting sites in terms of environment and Perkinsus olseni presence were chosen: Arguin (disease-free) and Ile aux Oiseaux (infected site). We monitored the dynamics of perkinsosis over the course of the experiment at Mundaka and Ile aux Oiseaux. Prevalences were high (>70%), and intensities were around 10(5) cells g(-1) wet gills at Ile aux Oiseaux, and 10(6) cells g(-1) at Mundaka. No significant differences in prevalence or... |
| Tipo: Text |
Palavras-chave: Clams; Perkinsus spp.; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Growth. |
| Ano: 2013 |
URL: https://archimer.ifremer.fr/doc/00166/27733/25951.pdf |
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| Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis. |
| Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1035.pdf |
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| Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Gueguen, Marielle; Moal, Jeanne; Palacios, Elena; Lassus, Patrick; Soudant, Philippe. |
| The effects of an artificial bloom of the toxin-producing dinoflagellate, Alexandrium minutum, upon physiological parameters of the Pacific oyster, Crassostrea gigas, were assessed. Diploid and triploid oysters were exposed to cultured A. minutum and compared to control diploid and triploid oysters fed T. Isochrysis. Experiments were repeated twice, in April and mid-May 2007, to investigate effects of maturation stage on oyster responses to A. minutum exposure. Oyster maturation stage, Paralytic Shellfish Toxin (PST) accumulation, as well as several digestive gland and hematological variables, were assessed at the ends of the exposures. In both experiments, triploid oysters accumulated more PSTs (approximately twice) than diploid oysters. Significant... |
| Tipo: Text |
Palavras-chave: Oysters; Ploidy; Physiology; Harmful-algal bloom; Alexandrium minutum; PST accumulation. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11378/7988.pdf |
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| Certain, Cassandre; Della Patrona, Luc; Gunkel-grillon, Peggy; Leopold, Audrey; Soudant, Philippe; Le Grand, Fabienne. |
| Cultivation of salt-tolerant crops help to face to irreversible global salinization of freshwater and soils. In New-Caledonia, three halophytes are candidates for saline crops, Salsola australis R.Br., Suaeda maritima (L.) Dumort and Enchylaena tomentosa R.Br. Their success and quality depend yet on availabilities of salinity and essential nutrients in agrosystems. So, we investigated effects of three salinities, i.e., control moderate and high, and five nitrogen ratios, i.e., 100:0, 75:25, 50:50, 25:75 or 0:100 NO3−-N:NH4+-N ratio on their growth and functional value for fatty acids and antioxidants. Results show that the leaf fatty acid and antioxidant profiles of species, emphasize their good potential to become functional crop products, based on... |
| Tipo: Text |
Palavras-chave: Halophyte; Salsola australis; Suaeda maritima; Enchylaena tomentosa; Salt tolerance; Nitrogen form; Fatty acid; Phenolic compounds; Antioxidant activity. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00682/79411/81960.pdf |
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| Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc. |
| In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potential (MMP), and intracellular ATP content in relation to motion parameters and 2) the involvement of OXPHOS for spermatozoal movement using carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Intracellular ATP content; Mitochondrial membrane potential; Oxidative phosphorylation; Sperm motility. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00303/41376/40666.pdf |
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| Miossec, Laurence; Arzul, Isabelle; Garcia, Celine; Soudant, Philippe; Francois, Cyrille; Leite, R. B.; Cancela, M. L.; De Blas, I.. |
| Infectious diseases in exploited shellfish population cause economical problems world-wide. The characteristics of the disease in the population -i.e. origin, frequency, distribution, development- need to be well known to set up appropriate measures for protection and restoration of shellfish population health. The investigation of disease in a population is the basis of epidemiology. Quantitative investigations include studies, survey, surveillance with specific sampling strategies according to the objectives. European clams (Ruditapes decussatus) and Manila clams (Ruditapes philippinarum), two commercially valuable species, are infected by the protozoan Perkinsus olseni. This pathogen has been associated with epizootic outbreaks involving in heavy... |
| Tipo: Text |
Palavras-chave: Recommendations; Epidemiology; Aquatic animal diseases; Pathology; Perkinsus olseni. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3326.pdf |
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| Stachowski-haberkorn, Sabine; Guesdon, Stephane; Bechemin, Christian; Chiffoleau, Jean-francois; Brach-papa, Christophe; Soudant, Philippe; Beker, Beatriz; Jadas-hécart, Alain. |
| TOPHYPAC is a field study that was conducted in the Charente river estuary (Marennes-Oléron bay). The main goal was to assess the possible impact of chemical contamination with copper and pesticides on phytoplankton communities. Physical, chemical (nutrients, metals and pesticides) and biological (phytoplankton community) parameters were monitored in the field during three years, from 2011 to 2014. In 2012 and 2013, field sampling was coupled with experimental exposure of phytoplankton communities to copper and to an herbicide cocktail, after transportation to the lab: the effects were assessed using photosynthesis endpoint and cellular densities. TOPHYPAC allowed to set the framework of Charente estuary contamination with pesticides: herbicides glyphosate... |
| Tipo: Text |
Palavras-chave: Continuum eau douce/eau côtière; Pesticides; Métaux; Communautés phytoplanctoniques; Efficacité de photosynthèse; PICT; Tolérance; Cytométrie en flux; Toxicité chronique; Freshwater/coastal water continuum; Pesticides; Metals; Phytoplankton communities; Photosynthesis efficiency; PICT; Tolerance; Flow cytometry; Chronic toxicity. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00300/41115/40289.pdf |
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| Dudognon, Tony; Guderley, Helga; Quere, Claudie; Soudant, Philippe; Racotta, Ilie; Kraffe, Edouard. |
| Although laboratory experiments allow greater control of environmental conditions than field studies, they have several drawbacks. To analyze physiological responses to forcing environmental variables, experimental conditions should mimic natural conditions as closely as possible. For filter-feeding organisms in particular, diet quality and quantity is one of the environmental parameters that can differ markedly between experimental and field conditions. In the hatchery, Pacific oysters, Crassostrea gigas, commonly show good physiological performance and growth on a mixed algal diet of Tisochrysis lutea, formerly Isochrysis aff. galbana clone Tahiti (T-Iso), and Chaetoceros calcitrans, presumably as it provides a good supply of essential polyunsaturated... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00266/37756/36065.pdf |
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| Hegaret, Hélène; Wikfors, Gary H.; Soudant, Philippe; Lambert, Christian; Shumway, Sandra E.; Berard, Jean-baptiste; Lassus, Patrick. |
| The possible effect of Alexandrium spp. containing paralytic shellfish poisoning (PSP) toxins on the hemocytes of oysters was tested experimentally. In one trial, eastern oysters, Crassostrea virginica Gmelin, were exposed to bloom concentrations of the sympatric dinoflagellate, Alexandrium fundyense Balech, alone and in a mixture with a non-toxic diatom, Thalassiosira weissflogii (Grun) Fryxell et Hasle. Subsequently, another experiment exposed Pacific oysters, Crassostrea gigas Thunberg, to a mixed suspension of the sympatric, toxic species Alexandrium catenella (Whedon et Kofoid) Balech, with T. weissflogii. Measurements of numbers of oyster hemocytes, percentages of different cell types, and functions (phagocytosis, reactive oxygen species (ROS)... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3328.pdf |
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| Paul-pont, Ika; Lacroix, Camille; Gonzalez Fernandez, Carmen; Hegaret, Helene; Lambert, Christophe; Le Goic, Nelly; Frere, Laura; Cassone, Anne-laure; Sussarellu, Rossana; Fabioux, Caroline; Guyomarch, Julien; Albentosa, Marina; Huvet, Arnaud; Soudant, Philippe. |
| The effects of polystyrene microbeads (micro-PS; mix of 2 and 6 μm; final concentration: 32 μg L−1) alone or in combination with fluoranthene (30 μg L−1) on marine mussels Mytilus spp. were investigated after 7 days of exposure and 7 days of depuration under controlled laboratory conditions. Overall, fluoranthene was mostly associated to algae Chaetoceros muelleri (partition coefficient Log Kp = 4.8) used as a food source for mussels during the experiment. When micro-PS were added in the system, a fraction of FLU transferred from the algae to the microbeads as suggested by the higher partition coefficient of micro-PS (Log Kp = 6.6), which confirmed a high affinity of fluoranthene for polystyrene microparticles. However, this did not lead to a modification... |
| Tipo: Text |
Palavras-chave: Microplastics; Fluoranthene; Mussel; Depuration; Oxidative system. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00344/45502/45848.pdf |
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| Van Der Meulen, Md; De Vriese, L; Lee, J; Maes, T; Van Dalfsen, Ja; Huvet, Arnaud; Soudant, Philippe; Robbens, J; Vethaak, Ad. |
| This report has been produced under the Interreg IVa project MICRO. We would like to thank the Joint Technical Secretariat of Interreg, the Dutch Ministry of Infrastructure and the Environment for co-financing the work conducted in this project. Also, the Institute for Environmental Studies of the VU University in Amsterdam and Stichting de Zeeschelp in Kamperland, many thanks for your analyses and experimental support and advice. Many thanks to all of the researchers who worked on this project, especially Christophe Lambert, David Mazurais, Rosanna Sussarellu, Ika Paul-Pont, Laura Frere, Anne-Laure Cassone, Dana Stuparu, Frank Kleissen, Ghada El Serafy, Sara Maes, Mattias Bossaer, Bavo De Witte, Johanna Gauquie, Caroline De Tender, Kevin Vanhalst. |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00287/39834/38359.pdf |
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| Lavaud, Romain; Artigaud, Sebastien; Le Grand, Fabienne; Donval, Anne; Soudant, Philippe; Flye-sainte-marie, Jonathan; Strohmeier, Tore; Strand, Oivind; Leynaert, Aude; Beker, Beatriz; Chatterjee, Arnab; Jean, Fred. |
| We combined the use of pigments, fatty acids and sterols as biomarkers of the seasonal variation in food sources of the great scallop Pecten maximus. From March to October 2011, on a biweekly to twice-weekly basis, scallops and seawater from the water column and the water-sediment interface were collected in the Bay of Brest (Brittany, France). Pigment compositions in the seawater and in the stomach and rectum content of the scallops were analyzed by HPLC. Fatty acids and sterols from digestive gland (DG) tissue were analyzed by gas chromatography. Potential relationships between the temporal proportion of each marker in the environment and in the digestive tract were tested using multivariate analysis. Proportions of diatoms and Dinophyceae biomarkers... |
| Tipo: Text |
Palavras-chave: Trophic ecology; Food sources; Pigments; Fatty acids; Trophic marker; Phytoplankton; Pecten maximus. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00431/54244/55772.pdf |
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| Tallec, Kevin; Huvet, Arnaud; Di Poi, Carole; Gonzalez-fernandez, Carmen; Lambert, Christophe; Petton, Bruno; Le Goic, Nelly; Berchel, Mathieu; Soudant, Philippe; Paul-pont, Ika. |
| In the marine environment, most bivalve species base their reproduction on external fertilization. Hence, gametes and young stages face many threats, including exposure to plastic wastes which represent more than 80% of the debris in the oceans. Recently, evidence has been produced on the presence of nanoplastics in oceans, thus motivating new studies of their impacts on marine life. Because no information is available about their environmental concentrations, we performed dose-response exposure experiments with polystyrene particles to assess the extent of micro/nanoplastic toxicity. Effects of polystyrene with different sizes and functionalization (plain 2-μm, 500-nm and 50-nm; COOH-50 nm and NH2-50 nm) were assessed on three key reproductive steps... |
| Tipo: Text |
Palavras-chave: Oyster; Embryos; Gametes; Microplastics; Nanoplastics. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00453/56419/58090.pdf |
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| Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Lambert, Christophe; Bideau, Antoine; Bidault, Adeline; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe. |
| The synthetic pathways responsible for the production of the polyunsaturated fatty acids 22:6n-3 and 20:5n-3 were studied in the Dinophyte Alexandrium minutum. The purpose of this work was to follow the progressive incorporation of an isotopic label (13CO2) into 11 fatty acids to better understand the fatty acid synthesis pathways in A. minutum. The Dinophyte growth was monitored for 54 h using high-frequency sampling. A. minutum presented a growth in two phases. A lag phase was observed during the first 30 h of development and had been associated with the probable temporary encystment of Dinophyte cells. An exponential growth phase was then observed after t30. A. minutum rapidly incorporated 13C into 22:6n-3, which ended up being the most 13C-enriched... |
| Tipo: Text |
Palavras-chave: PUFA synthesis; 22:6n-3; DHA; PKS pathway; Dinophyte; Alexandrium minutum; Encystment. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00654/76562/77688.pdf |
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| Foulon, Valentin; Le Roux, Frederique; Lambert, Christophe; Huvet, Arnaud; Soudant, Philippe; Paul-pont, Ika. |
| Microplastics collected at sea harbour a high diversity of microorganisms including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for potentially pathogenic microorganisms. In the present study we investigated the adhesion dynamics of Vibrio crassostreae on polystyrene microparticles (micro-PS) using electronic and fluorescence microscopy techniques. Micro-PS were incubated with bacteria in different media (Zobell culture medium and artificial seawater) with or without natural marine aggregates. The highest percentage of colonised particles (38-100%) was observed in Zobell culture medium, which may be related to nutrient availability for production of pili and exopolysaccharide adhesion structures.... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00351/46210/45908.pdf |
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| Delaporte, Maryse; Chu, Fu-lin; Langdon, Chris; Moal, Jeanne; Lambert, Christophe; Samain, Jean-francois; Soudant, Philippe. |
| The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana(T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50%... |
| Tipo: Text |
Palavras-chave: Polyunsaturated fatty acids; Immunology; Hemocytes; Eicosapentaenoic acid 20 : 5n 3; Crassostrea gigas; Bivalves. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2604.pdf |
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| Lassudrie, Malwenn; Soudant, Philippe; Nicolas, Jean-louis; Miner, Philippe; Le Grand, Jacqueline; Lambert, Christophe; Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline. |
| The Pacific oyster Crassostrea gigas is an important commercial species cultured throughout the world. Oyster production practices often include transfers of animals into new environments that can be stressful, especially at young ages. This study was undertaken to determine if a toxic Alexandrium bloom, occurring repeatedly in French oyster beds, could modulate juvenile oyster cellular immune responses (i.e. hemocyte variables). We simulated planting on commercial beds by conducting a cohabitation exposure of juvenile, “specific pathogen-free” (SPF) oysters (naïve from the environment) with previously field-exposed oysters to induce interactions with new microorganisms. Indeed, toxic Alexandrium spp. exposures have been reported to modulate bivalve... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Alexandrium; Harmful algal blooms; Hemocyte; Microbial challenge. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00314/42523/41894.pdf |
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| Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Guilpain, Patrice; Geairon, Philippe; Razet, Daniel; Seugnet, Jean-luc; Robert, Stephane; Ropert, Michel; Simonne, Charlotte; Hugonnet, Virginie; Moal, Jeanne; Pouvreau, Stephane; Huvet, Arnaud; Samain, Jean-francois; Lambert, Christophe; Soudant, Philippe; Frouel, Stephane; Choquet, Gwenaelle; Delaporte, Maryse; Paillard, Christine. |
| Four laboratories are involved in this multidisciplinary study: the Conchological Laboratory of Normandie (LCN), the Invertebrates Physiology Laboratory (LPI), the Université Bretagne Occidentale (LEMAR IHP) and the Conchological Laboratory of Poitou-Charentes (LCPC). The part of the study which is presented here (thematics "Coastal ecology") was produced in collaboration with the LCN. The data on the Bay of Veys was collected by Michel Ropert. This study aims at observing the "sites" effect on mortality and on the general biology of the animal, as well as measuring some environmental elements (e.g. water temperature, etc.). The geographical sites are Gefosse in the Bay of Veys (East Cotentin) and the Ronce bank in the south of the Marennes Oléron Bay. The... |
| Tipo: Text |
Palavras-chave: Immunologie; Physiologie; Paramètres environnementaux; Croissance; Ecologie côtière; Baies des Veys Cotentin; Ronce Charente Maritime; Comparaison sites; Reproduction; Mortalités; Crassostrea gigas; Huîtres. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/acte-3405.pdf |
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| Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Quere, Claudie; Bruneau, A; Riso, Ricardo; Auffret, Michel; Soudant, Philippe. |
| Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in... |
| Tipo: Text |
Palavras-chave: Harmful algae; Metals; Oysters; Toxin accumulation; Physiological effects. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00169/28071/26286.pdf |
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| Long, Marc; Paul-pont, Ika; Hegaret, Helene; Moriceau, Brivaela; Lambert, Christophe; Huvet, Arnaud; Soudant, Philippe. |
| To understand the fate and impacts of microplastics (MP) in the marine ecosystems, it is essential to investigate their interactions with phytoplankton as these may affect MP bioavailability to marine organisms as well as their fate in the water column. However, the behaviour of MP with marine phytoplanktonic cells remains little studied and thus unpredictable. The present study assessed the potential for phytoplankton cells to form hetero-aggregates with small micro-polystyrene (micro-PS) particles depending on microalgal species and physiological status. A prymnesiophycea, Tisochrysis lutea, a dinoflagellate, Heterocapsa triquetra, and a diatom, Chaetoceros neogracile, were exposed to micro-PS (2 μm diameter; 3.96 μg L−1) during their growth culture... |
| Tipo: Text |
Palavras-chave: Polystyrene; Microplastic; Aggregates; Microalgae; Marine; Phytoplankton. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00389/50016/50735.pdf |
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| Registros recuperados: 68 | |
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